Effectiveness of Cleaning Methods for Investigating Diatoms

M. Webber, J. van Dam and E.C. Humphrey*

Advanced Microscopy Facility, University of Victoria, CanadaDiatoms are routinely chemically cleaned of all organic matter in order to study the silicious wall (frustule) ultrastructure and to make accurate taxonomic determinations. Since the 1800‘s many methods have been developed for cleaning organic matter from diatoms [2, 6, 7]. Currently most techniques use concentrated acids, hydrogen peroxide or bleach. Recent developments in electron microscopy facilities include instruments for cleaning such as the plasma cleaner, UV (Zone) cleaner and sputtering by focused ion beam (FIB). Read more here

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